Simultaneous isolation of total cellular lipids and RNA from cultured cells

SIDR: simultaneous isolation and parallel sequencing of genomic DNA and total RNA from single cells.

Simultaneous sequencing of the genome and transcriptome at the single-cell level is a powerful tool for characterizing genomic and transcriptomic variation and revealing correlative relationships. However, it remains technically challenging to analyze both the genome and transcriptome in the same cell. Here, we report a novel method for simultaneous isolation of genomic DNA and total RNA (SIDR)...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

A universal and efficient protocol for the isolation of RNA from tissues and cultured cells.

This procedure is a modification of the original method published by Chirgwin et al. (1). The following protocol is especially adapted for small amounts of tissue and cells (1020mg wet weight) and results in undegraded RNA preparations, lg tissue or cells are homogenized with 8ml lysis buffer (4M guanidinium thiocyanate, 2% 2-mercaptoethanol, 0.1M Hepes pH 7.5, 0.5% (w/v) sarkosyl) using a moto...

Method for simultaneous RNA and DNA isolation from biopsy material, culture cells, plants and bacteria.

The analysis of RNA and DNA in clinical biopsy material for diagnostic and research purposes has become more and more important. Currently available methods and kits are used to focus on the extraction of only one kind of nucleic acid, but since the amount of biopsy material is often limited, a method for the simultaneous isolation of both kinds of nucleic acids from one sample is desirable. In...

An efficient method for isolation of RNA from tissue cultured plant cells.

Total cellular RNA has commonly been prepared from plant material utilizing methods involving CTAB (cetyltrimethylammonium bromide), SDS (sodium dodecyl sulfate), phenol/chloroform, or guanidine hydrochloride (Logemann et al, 1987). Although very effective in the preparation of intact RNA from leaf tissue, when applied to cultured plant cells these methods all result in a low yield and a substa...